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written 14.6 years ago by
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Comment: How to count the number and proportion of different barcode combinations in DNA
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I have no idea what you’re asking. “185 base sequences”? — i have no idea what that means; do you mean there are 185 possible barcodes? Whe…
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Show us a few examples of reads. Since you said `barcode` I assume these are `in-line` in the sequencing read. What is the length of these …
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Get a BED file of the promoter genomes and then use `bedtools intersect` (https://bedtools.readthedocs.io/en/latest/content/tools/intersect…
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A better option is to submit separate SLURM jobs for each genome. Using a `for` loop inside a single SLURM job is not efficient. Instead do…
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Thanks! I will check with him and see if this works for him.
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I understand how max-depth works, but I asked about max-idepth, indelgap and snpgap
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Start here: https://www.nextflow.io/
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Thank you so much, Dr. Lindenbaum, for the valuable comment. I'm not quite experienced in nextflow, but I like to test this out. Can you pr…
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> I cannot use it in anyway?
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I hope this may help:
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Pierre, thank you. When you say broken/corrupted does it mean I cannot use it in anyway? i was able to run samtools mpileup just this week …
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your file input.bam is broken/corrupted. There is nothing you can do but regenerating this file.
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Nice, but you'd better learn to use a workflow manager like snakemake or nextflow. See NF below (not tested):
```
workflow {
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I am very new to IGV I am not sure how to do that I took several screenshot because I do not know which part is the mutation I want
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