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1,000 results • Page
5 of 20
Sort: replies
Rank
Views
Votes
Replies
31
votes
34
replies
7.4k
views
11 follow
Forum:
pros- and cons- : programming skills vs. GUI
programming
lecture
updated 13 months ago by
Ram
43k • written 8.4 years ago by
TriS
★ 4.7k
80
votes
34
replies
5.3k
views
11 follow
Forum:
Authorship issues- how to resolve?
authorship
updated 16 months ago by
Ram
43k • written 8.9 years ago by
datanerd
▴ 520
41
votes
34
replies
16k
views
10 follow
Best tool for variant calling
SNP
genome
alignment
next-gen
updated 5.4 years ago by
claire
• 0 • written 5.9 years ago by
williamsbrian5064
▴ 510
54
votes
34
replies
4.9k
views
13 follow
Forum:
Should We Release Database Dumps Of All Questions On Biostar?
meta
biostars
updated 14 months ago by
Ram
43k • written 13.4 years ago by
Istvan Albert
100k
15
votes
34
replies
12k
views
Improving efficiency of awk for large files
awk
updated 18 months ago by
Ram
43k • written 8.8 years ago by
AW
▴ 350
2
votes
34
replies
6.8k
views
How to install pip on HPC server
software error
6.2 years ago by
Bioinfonext
▴ 460
12
votes
34
replies
5.1k
views
Hidden Markov Models Within Sequence Analysis -- Dispelling Misconceptions + Fixing Explanations
sequencing
updated 12.1 years ago by
Fabian Bull
★ 1.3k • written 12.6 years ago by
Delinquentme
▴ 200
44
votes
34
replies
13k
views
7 follow
Technical replicates in RNAseq
RNA-Seq
technical_replicates
2.2 years ago by
grant.hovhannisyan
★ 2.6k
16
votes
34
replies
4.8k
views
7 follow
GC percent detection for several number of genes
GC%
large number of genes
7.1 years ago by
nazaninhoseinkhan
▴ 520
30
votes
34
replies
6.7k
views
enrichment of gene set with enrichR
enrichR
RNA-Seq
R
updated 6.1 years ago by
WouterDeCoster
47k • written 6.1 years ago by
bikash2510
▴ 30
147
votes
34
replies
55k
views
17 follow
Tutorial:
Working with MAF files (Mutation Annotation Format) from the TCGA (The Cancer Genome Atlas)
music
cancer
tcga
mutation
maf
updated 14 months ago by
Ram
43k • written 11.0 years ago by
Cyriac Kandoth
6.0k
16
votes
34
replies
12k
views
6 follow
Why am I unable to load my data from a tab separated file into R?
microarray
programming
R
updated 2.0 years ago by
Ram
43k • written 9.2 years ago by
Mo
▴ 920
15
votes
34
replies
16k
views
10 follow
Issues installing bcl2fastq?
bcl2fastq
demultiplexing
updated 7 weeks ago by
barslmn
★ 2.1k • written 6.7 years ago by
a.rex
▴ 350
65
votes
34
replies
27k
views
20 follow
Phylogenetic Analysis Of Whole Genomes
phylogenetics
tree
updated 2.1 years ago by
Ram
43k • written 13.8 years ago by
Aparna
▴ 130
19
votes
34
replies
5.7k
views
6 follow
How can I convert the blast results into a matrix?
perl
linux
python
blast
updated 2.2 years ago by
Ram
43k • written 9.4 years ago by
kandoigaurav
▴ 150
172
votes
34
replies
26k
views
29 follow
Recommend Your Favorite Introductory "R In Bioinformatics" Books And Resources
r
books
bioconductor
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Michael
54k
121
votes
33
replies
39k
views
24 follow
Forum:
Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 11 months ago by
carlopecoraro2
★ 2.5k • written 10.7 years ago by
Eric Normandeau
11k
27
votes
33
replies
3.8k
views
7 follow
Forum:
Biostars question catagories
statistics
biostars
meta
updated 12 months ago by
Ram
43k • written 7.5 years ago by
John
13k
70
votes
33
replies
17k
views
18 follow
Choosing Random Set Of Seqs From Larger Set
random
fasta
updated 24 months ago by
Ram
43k • written 12.1 years ago by
razor
▴ 190
12
votes
33
replies
3.2k
views
Missing sequences in local NT database
nt
blast
ncbi
blastdbcmd
updated 5.6 years ago by
lieven.sterck
15k • written 5.6 years ago by
Prakki Rama
★ 2.7k
138
votes
33
replies
66k
views
19 follow
VCF files: Change Chromosome Notation
vcf
next-gen
sequence
updated 18 months ago by
Ram
43k • written 10.0 years ago by
Quak
▴ 490
27
votes
33
replies
17k
views
high % of reads mapped to multiple loci after STAR mapping
RNA-Seq
STAR
single-end
updated 6.4 years ago by
Hussain Ather
▴ 990 • written 6.4 years ago by
Lila M
★ 1.2k
50
votes
33
replies
55k
views
10 follow
I would like to add gene names to a volcano plot obtained from DEseq2
RNA-Seq
DEseq2
R
updated 6.0 years ago by
Kevin Blighe
87k • written 7.8 years ago by
ta_awwad
▴ 340
11
votes
33
replies
17k
views
How we can analyze sanger sequence chromatogram?
SNP
sequencing
alignment
sanger
updated 2.2 years ago by
Ram
43k • written 9.5 years ago by
Elnaaz
▴ 40
29
votes
33
replies
8.1k
views
12 follow
getBM error while using bioMart
biomart
getBM
ensembl
updated 3.6 years ago by
user17394
• 0 • written 3.6 years ago by
MatStat
▴ 160
41
votes
33
replies
18k
views
10 follow
How To Convert Xml Into A Decent Parseable Format?
xml
parsing
perl
subjective
updated 12.9 years ago by
Jrbeaman
▴ 30 • written 13.0 years ago by
Lyco
★ 2.3k
143
votes
33
replies
30k
views
20 follow
C/C++ Libraries For Bioinformatics?
c
c
updated 5.0 years ago by
chernouhov sergey
▴ 50 • written 13.9 years ago by
User 59
13k
24
votes
33
replies
8.5k
views
Contribute To The Nat Gen Paper "Principles For The Post-Gwas Functional Characterisation Of Risk Loci"
gwas
snp
updated 13.4 years ago by
Khader Shameer
18k • written 13.4 years ago by
Giovanni M Dall'Olio
28k
11
votes
33
replies
4.0k
views
Detect Regions In A List That Are Within 1 Megabase Of Each Other
python
snp
genomics
overlap
updated 12.6 years ago by
Michael
54k • written 12.6 years ago by
Rubal
▴ 350
48
votes
33
replies
43k
views
16 follow
Extract A Group Of Fasta Sequences From A File
fasta
python
perl
biopython
bioperl
updated 2.4 years ago by
Ram
43k • written 13.8 years ago by
Kyle
▴ 60
298
votes
33
replies
108k
views
25 follow
Tutorial:
Cheat Sheet For One-Based Vs Zero-Based Coordinate Systems
coordinates
updated 14 months ago by
Ram
43k • written 10.5 years ago by
Obi Griffith
20k
6
votes
33
replies
2.8k
views
I need to understand this image
phylogenies
updated 7.2 years ago by
Emily
23k • written 7.2 years ago by
eng_samar_zayed
• 0
10
votes
33
replies
3.9k
views
Looking for a tool like fastq screen but for ONT data
gridion
nanopore
ont
minion
updated 10 months ago by
Ram
43k • written 5.8 years ago by
Rox
★ 1.4k
16
votes
33
replies
8.4k
views
9 follow
Back-filling missing genotypes in merged VCF
vcf
updated 16 months ago by
Pierre Lindenbaum
161k • written 9.5 years ago by
Katie D'Aco
★ 1.1k
93
votes
32
replies
33k
views
26 follow
Linux Distros Best Suited For Bioinformatics?
linux
written 12.3 years ago by
Sat3Lite
▴ 150
88
votes
32
replies
9.6k
views
11 follow
Forum:
Vintage / unconventional pictures for Bioinformatics
history
picture
updated 2.1 years ago by
Ram
43k • written 9.5 years ago by
Pierre Lindenbaum
161k
32
votes
32
replies
8.0k
views
11 follow
Should We Be Using Much More Json In Our Delimited Data Formats?
gff
vcf
updated 13.2 years ago by
Erik Garrison
★ 2.4k • written 13.2 years ago by
Casbon
★ 3.3k
153
votes
32
replies
8.5k
views
23 follow
Picking A Programming Language And Where To Begin
programming
next-gen
sequencing
c
subjective
updated 12.2 years ago by
Palani Kannan
▴ 60 • written 12.7 years ago by
Travis
★ 2.8k
53
votes
32
replies
13k
views
13 follow
Bio-Javascript?
updated 2.0 years ago by
Ram
43k • written 13.2 years ago by
Lee Katz
★ 3.1k
3
votes
32
replies
3.4k
views
fastqc report for degradome reads
fastqc
adaptor
6.1 years ago by
Sam
▴ 150
82
votes
32
replies
7.7k
views
13 follow
Forum:
Shall We Go Back To Stackexchange?
meta
updated 14 months ago by
Ram
43k • written 11.4 years ago by
14134125465346445
★ 3.6k
9
votes
32
replies
3.0k
views
Differentially gene expressed analyses of two different samples
RNA-Seq
R
next-gen
rna-seq
updated 7.4 years ago by
EVR
▴ 610 • written 7.4 years ago by
Mehmet
▴ 820
0
votes
32
replies
9.4k
views
DoHeatmap clusters, Seurat pipeline
scRNA-sequencing
3.6 years ago by
Morris_Chair
▴ 350
129
votes
32
replies
129k
views
29 follow
Is There A Free Alternative To Ingenuity Pathway Analysis?
network
pathway
updated 2.1 years ago by
Ram
43k • written 13.2 years ago by
Gon
▴ 540
10
votes
32
replies
3.7k
views
6 follow
Why my CD31+ endothelial cells RNAseq didn't express CD31?
CD31
HISAT2
endothelialcells
Stringtie
RNAseq
3.0 years ago by
Youyy
• 0
9
votes
31
replies
5.0k
views
6 follow
Clustering Million Points : Transcript Start And End Sites
clustering
updated 12.0 years ago by
Khader Shameer
18k • written 12.1 years ago by
Abhi
★ 1.6k
59
votes
31
replies
4.9k
views
12 follow
News:
Example of how bioinformaticians can publish in Scientific Reports (by nature publishing group) using publicly available NGS data
publication
NGS
updated 12 months ago by
Ram
43k • written 7.3 years ago by
David Langenberger
11k
28
votes
31
replies
5.7k
views
8 follow
Forum:
Biostar Ads Are Now Live. Feedback And Comments Are Sought.
biostars
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Istvan Albert
100k
45
votes
31
replies
9.1k
views
6 follow
What Improvements Would You Recommend For This Genome Scaffolding Software?
genome
scaffolding
next-gen-sequencing
updated 21 months ago by
Ram
43k • written 13.1 years ago by
Michael Barton
★ 1.9k
38
votes
31
replies
2.8k
views
10 follow
Forum:
To the Biologists out there: What bioinf tool are you missing?
education
career
updated 12 months ago by
Ram
43k • written 7.8 years ago by
mschmid
▴ 180
1,000 results • Page
5 of 20
Recent Votes
Comment: NGS forensics: how to know if data is fabricated
A: Hard time trying to calculate Allele Frequency and DP from Platypus
Answer: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
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Answer: Differential Expression using Isoseq-supplemented reference transcriptome
by
Gordon Smyth
★ 7.0k
> What is the best method to test for differential abundance at the isoform level? Salmon returns TPM values instead of the counts which Li…
Answer: Finding batch and outlayers
by
Tigran
• 0
![enter image description here][1] [1]: /media/images/aa60b9ef-b08c-4093-9f0d-fb656a5c
Comment: NGS forensics: how to know if data is fabricated
by
benformatics
3.9k
Can you post an IGV screenshot of what this looks like?
Comment: NGS forensics: how to know if data is fabricated
by
benformatics
3.9k
Yeah some of the Illumina machines pre-trim adapters (not sure if this is still the standard). I've also definitely also seen quality-trimm…
Comment: PDB related issue
by
noodle
▴ 530
You can typically find much more useful info in the cif file, for example what you've found is called '_atom_site.pdbx_PDB_ins_code ' and d…
Comment: PDB related issue
by
Mensur Dlakic
★ 27k
The best way to find out is to read the original paper. My guess is that the residue has been crystallized in alternative conformations, wh…
Answer: NGS forensics: how to know if data is fabricated
by
Mensur Dlakic
★ 27k
I share the opinion by @philippbayer I have seen enough questionable things in life and in science to keep me permanently jaded, but there…
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
I downloaded the data in two files from the link you provided. With this command: megahit -1 ERR072246_1.fastq.gz -2 ERR072246_2.fastq…
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
If you have Linux the assembly should work on your system, but 8 GB is generally not enough for assembling larger genomes.
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
I think you might be getting stuck on less relevant parts of my exercise. The most important point was that nothing is wrong with the data.…
Comment: What does it mean single base resolution in sequencing?
by
dsull
★ 5.9k
Great comprehensive explanation and overview of the technologies -- with regards to sequencing assays, the one thing I'd add is SNV identif…
Answer: What does it mean single base resolution in sequencing?
by
dsull
★ 5.9k
I think that term means slightly different things in different contexts. For example, it's useful to profile mRNA modifications (e.g. m6a) …
Comment: Cannot process all the reads in a fast5 file?
by
chujie
• 0
Hi, I met the same problem, have you found the solution?
Answer: What does it mean single base resolution in sequencing?
by
LauferVA
4.2k
Hi @fe3e6f65 , In a single sentence, the answer to your question can be summarized as, "*the resolution of a genomic technology refers to …
Comment: PCA plot
by
LauferVA
4.2k
Without more context, we cannot interpret this PCA plot. There are many possibilities. In addition, this question is basic enough that it w…
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