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1,000 results • Page 2 of 20
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102
votes
44
replies
8.3k
views
22 follow
What Are Numbers Every Bioinformatician Should Know?
knowledge career
updated 7 months ago by 40k • written 10.1 years ago by 24k
128
votes
44
replies
30k
views
16 follow
Forum: Trimming adapter sequences - is it necessary?
NGS adapter RNA-Seq
updated 6 months ago by 40k • written 7.0 years ago by ★ 1.0k
59
votes
43
replies
19k
views
18 follow
Tutorial: Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign pacbio arrow smrt-limk
updated 6 months ago by 40k • written 6.0 years ago by ★ 1.4k
57
votes
43
replies
15k
views
8 follow
Forum: Best RNA-Seq aligner: A comparison of mapping tools
NGS alignment genome RNA-Seq sequence
updated 6 months ago by 40k • written 4.7 years ago by 10k
1
vote
43
replies
2.9k
views
Database from .gbk files
database gbk ncbi
updated 5 months ago by • 0 • written 3.3 years ago by ▴ 10
170
votes
43
replies
17k
views
25 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
updated 5 months ago by 11k • written 10.5 years ago by 98k
82
votes
43
replies
30k
views
26 follow
Tutorial: Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm rna-seq bioconductor
updated 3 months ago by ▴ 40 • written 9.9 years ago by ★ 7.7k
8
votes
42
replies
4.1k
views
NaS (Nanopore Synthetic-long) help
Assembly preprocessing
updated 8 months ago by 40k • written 8.3 years ago by ▴ 290
122
votes
42
replies
9.4k
views
21 follow
News: Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta biostars
updated 7 months ago by 40k • written 10.8 years ago by 98k
138
votes
42
replies
21k
views
22 follow
Forum: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
meta
updated 6 months ago by 3.7k • written 10.3 years ago by ★ 1.6k
13
votes
41
replies
1.9k
views
Is my file created completely
sam BWA-MEM BWA
updated 6 months ago by 40k • written 6.4 years ago by ▴ 10
203
votes
41
replies
24k
views
20 follow
Forum: Genomics is not Special. Computational Biologists are reinventing the wheel for big data biology analysis
genomics cram gatk galaxy
updated 7 months ago by 40k • written 8.9 years ago by ★ 5.2k
23
votes
41
replies
3.1k
views
6 follow
I want to correct the erroneous barcode file, and the Python code that I've written, using Biopython, is very slow. How can I make this process fast…
NGS biopython illumina WGS
updated 4 months ago by 40k • written 5 months ago by ▴ 30
98
votes
41
replies
5.0k
views
17 follow
Forum: I am really pissed off by the bioinformatics software world. Do/can we have a better solution?
software-error next-gen-sequencing
updated 5 months ago by 40k • written 7.1 years ago by ▴ 510
31
votes
41
replies
2.0k
views
12 follow
Forum: Question regarding journal publications
publications journals
updated 3 months ago by 40k • written 3.6 years ago by ▴ 40
170
votes
41
replies
91k
views
27 follow
Multiline Fasta To Single Line Fasta
fasta
updated 3 days ago by 40k • written 12.3 years ago by ▴ 250
26
votes
41
replies
15k
views
10 follow
WGCNA modules and categorical traits relationship
WGCNA
updated 10 months ago by • 0 • written 5.7 years ago by ▴ 120
18
votes
40
replies
17k
views
16 follow
Tutorial: Easy way to run easily orthoMCL (Copy & paste)
all-v-all orthomcl
updated 6 months ago by 40k • written 7.2 years ago by ▴ 170
6
votes
40
replies
9.8k
views
Fastx_Collapser Has No Fastq Output
fastx RNA-seq
updated 6 months ago by 40k • written 10.0 years ago by ▴ 240
151
votes
39
replies
23k
views
20 follow
Forum: A Farewell To Bioinformatics
bioinformatics
updated 7 months ago by 40k • written 10.7 years ago by 3.0k
25
votes
39
replies
5.0k
views
8 follow
Forum: Bioinformatics Study Group
study-group
updated 7 months ago by 40k • written 10.2 years ago by ▴ 440
69
votes
39
replies
3.8k
views
14 follow
Forum: Preparation for Bioinformatics.SE closing
meta biostars
updated 4 months ago by 40k • written 6.3 years ago by 13k
282
votes
39
replies
174k
views
29 follow
Tutorial: How to download raw sequence data from GEO/SRA
fastq SRA bam GEO
updated 6 months ago by 40k • written 9.1 years ago by 20k
81
votes
39
replies
6.6k
views
11 follow
News: State Of Biostar - Future Directions (January 2013)
biostars
updated 7 months ago by 40k • written 10.7 years ago by 98k
93
votes
39
replies
142k
views
19 follow
Extract Reads From A Bam File That Fall Within A Given Region
bam
updated 5 months ago by ▴ 140 • written 11.2 years ago by ▴ 380
36
votes
39
replies
3.1k
views
11 follow
Forum: Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast RNA-Seq alignment next-gen-sequencing
updated 4 months ago by 40k • written 5.9 years ago by ▴ 130
18
votes
39
replies
3.3k
views
Tool: CBioInfCpp.h as a C++ lib containing some functions for bioinformatics
cpp
updated 4 months ago by 40k • written 4.5 years ago by ▴ 50
100
votes
38
replies
29k
views
34 follow
Forum: List of cloud genomics companies
cloud-genomics
updated 26 days ago by 21k • written 9.9 years ago by ★ 3.6k
129
votes
38
replies
6.7k
views
21 follow
Forum: Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics
updated 3 months ago by 40k • written 4.5 years ago by 98k
229
votes
38
replies
140k
views
29 follow
Tool: Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
ensembl genome-coordinates liftover
updated 3 months ago by 40k • written 10.6 years ago by 19k
48
votes
38
replies
16k
views
8 follow
Forum: Eukaryotic Genome Annotation in 2016
software genome annotation
updated 5 months ago by 40k • written 7.5 years ago by 11k
47
votes
37
replies
7.9k
views
12 follow
Forum: The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 7 months ago by 40k • written 10.1 years ago by 2.3k
43
votes
37
replies
1.9k
views
7 follow
Forum: Checking the quality and reliability in bioinformatics
reproducibility
updated 4 months ago by 40k • written 6.0 years ago by ★ 4.2k
110
votes
37
replies
7.4k
views
13 follow
Bioinformatics Career Survey 2011/2012
career
updated 7 months ago by 40k • written 12.0 years ago by 157k
244
votes
36
replies
8.5k
views
32 follow
Forum: Good Habit for Bioinformatics Analyst or Scientist
Analyst Scientist
updated 5 months ago by 40k • written 7.4 years ago by ★ 9.3k
49
votes
36
replies
7.8k
views
11 follow
Forum: New features and bug fixes for Biostar to work on during Google Summer of Code
meta biostars gsoc
updated 7 months ago by 40k • written 9.4 years ago by ▴ 110
251
votes
36
replies
32k
views
27 follow
Forum: How Do You Manage Your Files & Directories For Your Projects ?
project-management file-management
updated 6 months ago by 40k • written 13.4 years ago by 157k
9
votes
36
replies
11k
views
18 follow
Convert Gene IDs of Tomato Sol Database
tomato gene
updated 9 months ago by 40k • written 8.2 years ago by ▴ 170
17
votes
36
replies
3.7k
views
6 follow
GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
R
updated 10 months ago by ★ 1.8k • written 2.9 years ago by ▴ 60
149
votes
35
replies
60k
views
33 follow
Tutorial: Tools For Metagenomic Data Analysis
metagenomics next-gen
updated 7 months ago by 40k • written 10.8 years ago by ★ 1.6k
4
votes
35
replies
8.7k
views
Is They Any Software Or Technique To Find The Number Of Reads Mapped To Each Contig After Aligment
mapping RNA-seq
updated 6 months ago by 40k • written 9.7 years ago by ▴ 50
40
votes
35
replies
12k
views
10 follow
find positions of a short sequence in a genome
sequence search
updated 6 weeks ago by ★ 1.3k • written 7.1 years ago by 13k
79
votes
35
replies
8.5k
views
17 follow
Forum: Do bioinformaticians often break molecular biologists' hearts by being the first author?
authorship career
updated 4 months ago by • 0 • written 7.9 years ago by ▴ 650
116
votes
35
replies
7.6k
views
18 follow
Forum: Is Biostar Killing The Bioinformatics Core?
core biostars
updated 7 months ago by 40k • written 10.5 years ago by 21k
5
votes
35
replies
2.6k
views
Reduce set of chromosomes in Pangenome graph
pangenome vg
updated 11 weeks ago by ▴ 360 • written 3 months ago by • 0
76
votes
35
replies
28k
views
13 follow
Illumina Instrument Type from fastq?
fastq
updated 4 months ago by ▴ 20 • written 7.3 years ago by 6.5k
121
votes
35
replies
40k
views
20 follow
Forum: Snakemake vs. Nextflow: strengths and weaknesses
snakemake nextflow
updated 4 months ago by 40k • written 6.3 years ago by ▴ 790
26
votes
35
replies
30k
views
16 follow
Calculate The Frequency Of Nucleotides At Each Position In An Mpileup File
mpileup
updated 7 months ago by ▴ 10 • written 9.5 years ago by ★ 4.1k
83
votes
34
replies
17k
views
22 follow
Tutorial: Enrichment Analysis, Clustering and Scoring with pathfindR
enrichment R pathway active-subnetwork
updated 4 months ago by • 0 • written 5.3 years ago by ★ 1.3k
36
votes
34
replies
2.2k
views
9 follow
Forum: New to bioinformatics.....
gene
updated 4 months ago by 40k • written 5.5 years ago by • 0
1,000 results • Page 2 of 20
Recent Votes
Comment: ATAC-seq troubleshoot - Just Noise
t2t human reference genome for RNA-seq
Answer: Samtools Failing to Install through Conda
Answer: Importance of Data Structures for Bioinformatics?
Answer: Filter transcription factors
Comment: Low Alignment rate
Comment: Understanding TCGA barcodes with dot in the fieldname
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Recent Replies
Comment: ATAC-seq troubleshoot - Just Noise by vk ▴ 40
Do you think there's a possibility that something may have gone wrong in the sequencing step, as the bio-analyzer run seems to look okay or…
Answer: Samtools Failing to Install through Conda by hukai916 ▴ 10
Same issue on brand new M2 chip MacBook: conda seems NOT able to find `samtools`; `brew` worked.
Comment: Enrichment of mitochondrial and ribosomal pathways - an artifact? by LauferVA 3.7k
im unclear on why you would ask this question without providing more context about the cell states that are being explored by the experimen…
Comment: Should I Learn Docker to Run Command Line Bioinformatics Tool? by Ram 40k
Singularity is a good option but OP explicitly mentions that their users are using their own laptops. On laptops, it's a lot easier to use …
Comment: Calculation of TMB on gene level by Zhenyu Zhang ▴ 980
It's what you asked for just mutation frequency or mutation frequency adjusted by gene length?
Comment: Understanding TCGA barcodes with dot in the fieldname by Zhenyu Zhang ▴ 980
second this. The "95FC84F5-4FB3-4CE7-ACD0-C7E0D8F03DD7" is obviously an UUID, not related to TCGA barcode
Comment: Should I Learn Docker to Run Command Line Bioinformatics Tool? by Yogi ▴ 10
Just 1 thing to consider. `Docker` is great because it provides a way to containerize applications. HOWEVER, many people on computation…
Comment: Filter human transcription factors by Yogi ▴ 10
Definitely the best resource. Was also going to recommend this one.
Comment: GO analysis after DESeq2 by Ram 40k
The go category needs to be a [facet][1] on the ggplot. [1]: http://www.sthda.com/english/wiki/ggplot2-facet-split-a-plot-into-a-matrix-…
Comment: How to sort cd-hit-est cluster file by Mo ▴ 40
Hi, Thank you so much, this has worked :)
Comment: Program for Overlapping DMRs (Differentially Methylated Regions) Between Groups by LChart 3.3k
You might specify how the data is formatted. If you have `chr start end` columns, you can use `bedtools` to perform the interval operations.
Comment: RepeatMasker error when trying to generate repeat sequence distribution pie char by epianalysis • 0
Do you mind telling me how you were able to create a custom txdb object that sufficiently annotates repeats?
Comment: obtaining circular RNAs' sequences from circBase by aminijavad4 ▴ 10
I didn't know
Comment: obtaining circular RNAs' sequences from circBase by aminijavad4 ▴ 10
Thank you for your reply, Barry. Upon further searching, I discovered that circular RNA sequences can be downloaded from the downloads sect…
Answer: Saving the output of LD pruning from SNPRelate package as a new GDS file by James W. MacDonald ▴ 20
You want `createDataFile` from `GWASTools`. Ideally you wouldn't do that though - it takes forever to create a new GDS file, and the whole …
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