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Generally these genes are highly expressed, so there would be some sort of a power bias. How do you do the DE analysis? Any cutoffs on mini…
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You can run `agat_sp_compare_two_annotations.pl` from [AGAT][1], you should get the information you are looking for. This method allow to c…
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Thank you for the fast help, I have the BBMap suite installed anyways and will try it out :)
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yes got it, thank you very much.
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@swbarnes2, thanks for the response, by the way, can you tell me why/ how this happens during the sequencing process, and if it could negat…
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> can you explain this ??
yes. you should use options like `-u` .
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so validate the answer to close the question please (Green tick on the left).
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hi Pierre Linderbaum,
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please, comment or validate the answers to your previous questions: https://www.biostars.org/p/9573151/#9573158 ; https://www.biostars.org/…
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extract genes (e.g: awk '($3=="gene") {printf("%s\t%d\t%s\n",$1;int($4)-1,$5);}' ) from both files n sort, and use bedtools intersect with…
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G is the letter you get if there is no fluorescence.
75% of your reads have a lot of G.
I don't think this run worked.
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