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355 results • Page
3 of 8
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Views
Votes
Replies
10
votes
6
replies
2.2k
views
Forum:
How to acknowledge in the paper a reference genome that hasn't been published yet?
reference
paper
updated 12 months ago by
Ram
43k • written 5.8 years ago by
ciemanek
▴ 140
19
votes
14
replies
2.2k
views
Forum:
What is the amount of sequencing data produced annually?
data
research
sequencing
updated 8 months ago by
Jeremy Leipzig
22k • written 8 months ago by
vincenthus
▴ 70
31
votes
16
replies
2.2k
views
8 follow
Forum:
What is in your opinion the current state-of-the-art sequencing platform for genomic assembly (Nov 2019)
next-gen-sequencing
assembly
genome
updated 11 months ago by
Ram
43k • written 4.6 years ago by
Antonio R. Franco
★ 5.1k
15
votes
9
replies
2.2k
views
6 follow
Forum:
[Opinion] - What alignment software do you use?
next-gen
alignment
updated 12 months ago by
Ram
43k • written 6.1 years ago by
Andrew_MacGregor
▴ 30
26
votes
27
replies
2.2k
views
10 follow
Forum:
Determining computer time required
computer
hpc
time
updated 4 months ago by
dsull
★ 6.0k • written 4 months ago by
Rozita
▴ 40
7
votes
4
replies
2.2k
views
Forum:
How hard it is to get a bioinformatics degree in one country but find a career in another country?
career
Masters
updated 12 months ago by
Ram
43k • written 6.0 years ago by
dominic.dong
▴ 10
10
votes
15
replies
2.2k
views
Forum:
How should reference genome fasta files be distributed by UCSC?
reference-genome
fasta
freeze
ucsc-genome-browser
updated 11 months ago by
Jeremy Leipzig
22k • written 15 months ago by
Maximilian Haeussler
★ 1.6k
14
votes
10
replies
2.2k
views
Forum:
WGBS alignment with BitMapperBS
BitMapperBS
bismark
updated 11 months ago by
Ram
43k • written 4.8 years ago by
Shicheng Guo
★ 9.4k
3
votes
6
replies
2.2k
views
Forum:
Choosing the appropriate test for differential analysis: Limma or Mann-Whitney (with BH correction)? Test for normality
R
Shapiro-Wilk
Limma
Mann-Whitney
updated 11 months ago by
Ram
43k • written 4.0 years ago by
innyus
▴ 40
3
votes
14
replies
2.1k
views
6 follow
Forum:
Search for gene variants
next-gen
updated 12 months ago by
Ram
43k • written 5.7 years ago by
Rm
8.3k
16
votes
8
replies
2.1k
views
8 follow
Forum:
What are the most important unresolved problems in Bioinformatics?
bioinformatics
updated 10 months ago by
Ram
43k • written 7.6 years ago by
chen
★ 2.5k
49
votes
20
replies
2.1k
views
10 follow
Forum:
First experiences matter
first-time
tolerance
updated 4 months ago by
Michael Love
★ 2.6k • written 4 months ago by
Mensur Dlakic
★ 27k
21
votes
15
replies
2.1k
views
9 follow
Forum:
Working abroad without a PhD
career
updated 11 months ago by
Ram
43k • written 4.7 years ago by
Rox
★ 1.4k
23
votes
11
replies
2.1k
views
9 follow
Forum:
file and directory management best practices
file-management
updated 11 months ago by
Ram
43k • written 3.8 years ago by
nkinney06
▴ 140
0
votes
6
replies
2.1k
views
7 follow
Forum:
Up-to-date RNA-Seq Analysis Training/Courses/Papers
software-error
updated 12 months ago by
Ram
43k • written 5.7 years ago by
kullboys
• 0
1
vote
8
replies
2.1k
views
Forum:
Memory-driven computing accelerates genomic data processing
HPC
updated 11 months ago by
Ram
43k • written 5.3 years ago by
5heikki
11k
19
votes
15
replies
2.1k
views
6 follow
Forum:
Cross out line in Biostars
meta
updated 12 months ago by
Ram
43k • written 6.0 years ago by
Bastien Hervé
5.3k
21
votes
11
replies
2.1k
views
Forum:
Most important International Big Data Projects: Genetics and Epigenetics
big-data
updated 12 months ago by
Ram
43k • written 5.8 years ago by
Shicheng Guo
★ 9.4k
7
votes
7
replies
2.0k
views
Forum:
Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career
advice
updated 4 weeks ago by
Dunois
★ 2.5k • written 4 weeks ago by
AdviceSeeker
• 0
10
votes
7
replies
2.0k
views
Forum:
Is trimming always better?
RNA-Seq
QC
trimmomatic
trimming
updated 11 months ago by
Ram
43k • written 4.9 years ago by
Mozart
▴ 330
7
votes
8
replies
2.0k
views
Forum:
Would solving many Rosalind problems show your ability for this field in job-wise?
rosalind
career
11 months ago by
K
• 0
7
votes
4
replies
2.0k
views
Forum:
Bioinformatics / Computational Biology PhD
Computational-Biology
bioinformatics
PhD
updated 10 months ago by
Ram
43k • written 8.2 years ago by
MaLMat
• 0
5
votes
8
replies
2.0k
views
7 follow
Forum:
Ranking of Bioinformatics tools & resources by the number of users
bioinformatics-tools
updated 12 months ago by
Ram
43k • written 5.7 years ago by
BioICoder
▴ 40
17
votes
17
replies
2.0k
views
6 follow
Forum:
Journal for beginners
journal
updated 12 months ago by
Ram
43k • written 6.2 years ago by
heref
▴ 20
23
votes
12
replies
2.0k
views
6 follow
Forum:
Whole-Exome Analysis on a Mainstream Desktop PC
NGS
whole-exome
updated 11 months ago by
Ram
43k • written 3.9 years ago by
David Langenberger
11k
22
votes
8
replies
2.0k
views
10 follow
Forum:
Is there any online course for bioinformatics right now or in the next two or three months?
online-course
mooc
updated 11 months ago by
Ram
43k • written 4.1 years ago by
Eugenie
▴ 50
9
votes
6
replies
2.0k
views
6 follow
Forum:
GTF files from Ensembl Releases 105 and 106 unsorted
Ensembl
bug
GTF
updated 8 months ago by
ATpoint
82k • written 18 months ago by
dlaehnemann
▴ 30
12
votes
0
replies
2.0k
views
Forum:
Free Bioinformatics Courses(Curriculum)
ossu
course
Curriculum
updated 11 months ago by
Ram
43k • written 4.6 years ago by
Leite
★ 1.3k
17
votes
14
replies
1.9k
views
10 follow
Forum:
What would be the trend in next few years in NGS era?
NGS
sequencing
updated 11 months ago by
Ram
43k • written 5.5 years ago by
ahmad mousavi
▴ 800
6
votes
5
replies
1.9k
views
Forum:
Stanford HCP MS Biomedical Informatics vs Johns Hopkins MS Bioinformatics
career
updated 11 months ago by
Ram
43k • written 4.3 years ago by
melissachua90
▴ 70
7
votes
4
replies
1.9k
views
Forum:
I'm currently in my second year of my phd, I'm intrested to develop somthing for galaxy server
workflow
galaxy
updated 12 months ago by
Ram
43k • written 5.6 years ago by
dego999
• 0
3
votes
4
replies
1.9k
views
Forum:
Recommendation for applied bioinformatics labs focusing on cancer biology
cancer-genomics
updated 12 months ago by
Ram
43k • written 5.7 years ago by
Wenhu_Cao
▴ 100
0
votes
3
replies
1.9k
views
Forum:
European Genome-phenome Archive (EGA) vs Gene Expression Omnibus (GEO)
GEO
EGA
updated 11 months ago by
Ram
43k • written 5.2 years ago by
Shicheng Guo
★ 9.4k
18
votes
16
replies
1.9k
views
Forum:
Why are people not working on gene prioritization and candidate gene selection for new experiment design?
gene-prioritization
updated 12 months ago by
Ram
43k • written 5.7 years ago by
prasadhendre
▴ 20
22
votes
12
replies
1.8k
views
6 follow
Forum:
What were the most impactful Bioinformatics papers published this year
papers
publications
updated 11 months ago by
Ram
43k • written 4.4 years ago by
c_u
▴ 520
7
votes
10
replies
1.8k
views
Forum:
Current MCB major thinking of going into Bioinformatics
career
updated 12 months ago by
Ram
43k • written 5.8 years ago by
SeYu
• 0
7
votes
6
replies
1.8k
views
Forum:
Bioinformaticians - Am I on the Right Track?
Career
updated 11 months ago by
Ram
43k • written 4.5 years ago by
kstangline
▴ 80
8
votes
4
replies
1.8k
views
Forum:
Unsolved Topics in Bioinformatics
bioinformatics
updated 12 months ago by
Ram
43k • written 6.1 years ago by
BioICoder
▴ 40
1
vote
4
replies
1.8k
views
Forum:
What do you want to learn about bioinformatics?
course
education
updated 11 months ago by
Ram
43k • written 5.4 years ago by
AlexAbdulkaderKheirallah
▴ 120
21
votes
18
replies
1.8k
views
Forum:
Is it safe to keep our data in github
github
updated 11 months ago by
Ram
43k • written 4.0 years ago by
rprog008
▴ 70
7
votes
5
replies
1.8k
views
Forum:
Which is better: webgestalt vs david?
webgestalt
david
updated 11 months ago by
Ram
43k • written 5.2 years ago by
Shicheng Guo
★ 9.4k
4
votes
4
replies
1.7k
views
Forum:
When will the hg19/grch37 finally become obsolete?
Reference-Genome
updated 11 months ago by
Ram
43k • written 5.0 years ago by
gdaly9000
▴ 10
9
votes
10
replies
1.7k
views
Forum:
Brainstorming: applying bioinformatics NOT to public field. Ideas and perspectives
applied-sciences
data-analysis
updated 12 months ago by
Ram
43k • written 6.1 years ago by
lessismore
★ 1.3k
6
votes
15
replies
1.7k
views
Forum:
PhD Bioinformatics on GWAS
SNP
GWAS
genome
PHD
updated 11 months ago by
Ram
43k • written 4.3 years ago by
davidenoma
▴ 50
6
votes
3
replies
1.7k
views
Forum:
lincRNA analysis pipeline and relevant database
lncRNA
updated 12 months ago by
Ram
43k • written 5.6 years ago by
Shicheng Guo
★ 9.4k
4
votes
13
replies
1.7k
views
Forum:
Looking for Participants: Meenta NovaSeq RunShare Beta
RunShare
next-gen
NovaSeq
updated 11 months ago by
Ram
43k • written 4.8 years ago by
Torrey Ah-Tye
▴ 10
2
votes
8
replies
1.7k
views
7 follow
Forum:
Which tools do you miss?
software
updated 11 months ago by
Ram
43k • written 3.8 years ago by
ete
▴ 110
11
votes
8
replies
1.7k
views
Forum:
Bioinformatics cores pricing model
pricing-models
core
Bioinformatics-services
updated 11 months ago by
Ram
43k • written 5.2 years ago by
Bioinfo_2006
▴ 160
1
vote
9
replies
1.7k
views
Forum:
Care to speculate? Are Protein Fragments or Entire Protein Sequences useful when classifying via Machine Learning techniques
protein
Machine-Learning
classification
updated 11 months ago by
Ram
43k • written 5.3 years ago by
mcc
▴ 80
26
votes
8
replies
1.7k
views
7 follow
Forum:
Reasons to refuse to give support
design
rna-seq
statistics
updated 11 months ago by
Ram
43k • written 5.5 years ago by
Benn
8.3k
355 results • Page
3 of 8
Recent Votes
Post-doctoral Position in Alzheimer’s Disease Genetics, ‘Omics, and Biomarkers
The Biostar Handbook. A bioinformatics e-book for beginners.
Answer: How to add Ensembl id to cluster.markers in Seurat
How to add Ensembl id to cluster.markers in Seurat
Comment: Duplicated sequence samtools
Answer: Duplicated sequence samtools
Answer: Duplicated sequence samtools
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▴ 30
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Recent Replies
Comment: Is it Possible to "Merge" data from Replicates within a VCF SNP File
by
Pierre Lindenbaum
161k
> Cannot read non-existent file: file:///local/home/User/VCF_Filtering/biostar.code your file doesn't exist, is at the wrong place etc.
Comment: Is it Possible to "Merge" data from Replicates within a VCF SNP File
by
ajbarrett98
• 0
Hi Pierre, I am attempting to run this code and am running into this error: (jvar) $ jvarkit vcffilterjdk -f biostar.code Dart_VCF2_Or…
Comment: scRNA-seq data trained model can be used for predictions on bulk RNA-seq data?
by
ATpoint
82k
I cannot give a precise answer, as ML is not my field, but generally, why don't you apply the method to pseudobulks of the exact single-cel…
Answer: geom_bar issue in ggplot2 with xlim()
by
ATpoint
82k
https://stackoverflow.com/questions/10365167/geom-bar-bars-not-displaying-when-specifying-ylim
Comment: What marks a De-Novo Genome assembly as FAILED?
by
Umer
▴ 50
Yes. We are sequencing 45 different samples with illumina. 12 of these are also going to be sequenced with Nanopore (for hybrid assembly). …
Comment: HGVS Nomenclature of Multiple Indels found in Cis
by
LauferVA
4.2k
how has this code progressed? is it publically available? would you want to work together?
Comment: Design for complex RNA-Seq experiment using Deseq2
by
Ezequiel
• 0
I completely agree with most points. The PCA shows that the biggest driver of difference is the patients themselves, with PRE and POST time…
Comment: Add stats to the plot
by
marco.barr
▴ 130
Hi, you can add statistical annotations using `ggpubr` function `stat_pvalue_manual `. You need to make sure you calculate the p-values fir…
Answer: seqtk subseq in.fastq list.txt > out.fastq not extracting full sequence from
by
ATpoint
82k
Use everything before the whitespace in list.txt, for example `A00869:294:HCW57DSXY:2:1101:3929:1000` and not `A00869:294:HCW57DSXY:2:1101:…
Answer: CellRanger output more cells than specified using --force-cells? Why?
by
scideas
▴ 30
I actually had this same issue with CellRanger v7- turns out that since you have 2 species in your mapping reference, CellRanger is forcing…
Answer: Checking chromosome builds for genotyping data
by
Muhammad
• 0
Assume you have genotype data in Plink format. (G.bed, G.bim, G.fam) Download some reference panel data in Plink format (R.bed, R.bim, R.f…
Comment: Error in CIBERSORTx ($ operator is invalid for atomic vectors)
by
nguyenn6
• 0
did you ever figure it out? i'm having the same issue.
Comment: Traveler with Infernal mapping failed
by
anton.i.petrov
• 0
Hi Larissa! I am the lead developer of R2DT and I've just noticed this question. Could you please email help@r2dt.bio with your input seque…
Comment: constructing pangenome through psvcp
by
analyst
▴ 50
Fixed the issue by updating nucmer version.
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Can you create a small test database (`makeblastdb`) from your query file and try to run the search against the same file and see if that w…
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