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1,000 results • Page
4 of 20
Sort: Views
Rank
Views
Votes
Replies
128
votes
44
replies
30k
views
16 follow
Forum:
Trimming adapter sequences - is it necessary?
NGS
adapter
RNA-Seq
updated 6 months ago by
Ram
40k • written 7.0 years ago by
Lars
★ 1.0k
34
votes
11
replies
30k
views
6 follow
Sjdboverhang Option In Star
updated 12 weeks ago by
Kermit
▴ 80 • written 9.6 years ago by
Martombo
★ 3.1k
84
votes
29
replies
30k
views
20 follow
Tutorial:
How to create a mutation landscape (waterfall) plot with GenVisR
BioConductor
GenVisR
Graphics
R
updated 9 weeks ago by
amziepickles
• 0 • written 7.6 years ago by
Obi Griffith
20k
5
votes
6
replies
30k
views
Computing fold change values for RT-PCR
RT-PCR
Fold-change
updated 9 months ago by
Ram
40k • written 8.3 years ago by
Zara
▴ 20
10
votes
7
replies
29k
views
How To Batch Download Journal Papers (Pdf)?
papers
updated 6 months ago by
Ram
40k • written 11.3 years ago by
Hranjeev
★ 1.5k
14
votes
8
replies
29k
views
8 follow
How to read vcf file in python?
vcf
reader
python
updated 7 months ago by
BCArg
▴ 70 • written 3.7 years ago by
ja4123
▴ 10
19
votes
10
replies
29k
views
6 follow
read depth using samtools
depth
samtools
updated 16 days ago by
Sachin
• 0 • written 4.7 years ago by
LimMo
▴ 30
48
votes
18
replies
29k
views
16 follow
extract only geneID and gene symbol from GTF file
gtf
python
genome
updated 7 months ago by
Ram
40k • written 8.4 years ago by
catherine
▴ 250
17
votes
14
replies
29k
views
13 follow
Merge Paired-End Reads
fastq
updated 5 months ago by
Ram
40k • written 12.0 years ago by
Nicolas Rosewick
10k
24
votes
11
replies
29k
views
9 follow
Bed For Agilent Sureselect All Exon Kits ?
bed
exome
agilent
next-gen
written 10.9 years ago by
Pierre Lindenbaum
157k
20
votes
10
replies
29k
views
Converstion of BED with scores into bigWig
bed
bigWig
updated 10 months ago by
Ram
40k • written 8.2 years ago by
Leszek
4.2k
9
votes
12
replies
29k
views
6 follow
How To Download Gene Sequences From Ncbi Gene
ncbi
fasta
gene
updated 6 months ago by
Ram
40k • written 11.8 years ago by
Pierre
▴ 20
100
votes
38
replies
29k
views
34 follow
Forum:
List of cloud genomics companies
cloud-genomics
updated 1 day ago by
Jeremy Leipzig
21k • written 9.9 years ago by
14134125465346445
★ 3.6k
50
votes
15
replies
29k
views
10 follow
Where To Download Pam50 Gene Set?
cancer
annotation
classification
updated 11 weeks ago by
DareDevil
★ 3.3k • written 10.2 years ago by
user
▴ 940
26
votes
7
replies
29k
views
Is Paired End analysis possible with FASTQC?
dna-seq
QC
paired-end
fastqc
updated 8 months ago by
Ram
40k • written 7.4 years ago by
chefarov
▴ 160
36
votes
16
replies
28k
views
14 follow
Generating consensus sequence from bam file
genome
samtools
bwa
fasta
updated 10 days ago by
jkbonfield
★ 1.1k • written 4.6 years ago by
chparada
▴ 60
76
votes
35
replies
28k
views
13 follow
Illumina Instrument Type from fastq?
fastq
updated 5 months ago by
Nathan
▴ 20 • written 7.3 years ago by
andrew.j.skelton73
6.5k
28
votes
14
replies
28k
views
10 follow
Number Of Reads In A Sam File Which Are Assigned To Each Chromosome?
alignment
sam
updated 10 months ago by
Ram
40k • written 11.9 years ago by
KCC
★ 4.1k
31
votes
12
replies
28k
views
7 follow
Tutorial:
So What Does The Sequence Duplication Rate Really Mean In A Fastqc Report
quality-control
fastqc
updated 7 months ago by
Ram
40k • written 10.0 years ago by
Istvan Albert
98k
298
votes
122
replies
28k
views
29 follow
Mean Length Of Fasta Sequences
code
fasta
sequence
codegolf
updated 25 days ago by
jena
▴ 270 • written 13.2 years ago by
Eric Normandeau
11k
14
votes
8
replies
28k
views
NarrowPeak format of ChiP-seq
ChIP-Seq
tag-density
updated 3 months ago by
minakshiboruahassam
• 0 • written 9.3 years ago by
liu4gre
▴ 210
24
votes
12
replies
28k
views
8 follow
Sort VCF File by Position?
chromosome
vcf
updated 7 months ago by
ATpoint
77k • written 5.6 years ago by
Niell
▴ 20
15
votes
8
replies
28k
views
Input GSEA Pre-ranked list
gene
next-gen
RNA-Seq
genome
software
updated 9 months ago by
Ram
40k • written 8.2 years ago by
stevenlang123
▴ 200
19
votes
9
replies
28k
views
7 follow
Can anyone suggest a good tutorial to learn RNA-seq analysis?
analysis
RNA-Seq
ngs
updated 4 months ago by
Ram
40k • written 5.9 years ago by
Arindam Ghosh
▴ 510
99
votes
8
replies
28k
views
8 follow
Tutorial:
Guide to TCGA data
cancer
RNA-Seq
clinical
TCGA
updated 5 months ago by
Ram
40k • written 7.6 years ago by
Jordan Anaya
★ 1.1k
9
votes
5
replies
28k
views
7 follow
DESeq2 analysis for multiple conditions
RNA-Seq
DESeq2
updated 8 months ago by
Ram
40k • written 8.3 years ago by
eager_learner
▴ 60
17
votes
15
replies
28k
views
9 follow
Mapping Mouse gene names to Human gene names
RNA-Seq
PDX
updated 9 months ago by
Ram
40k • written 8.3 years ago by
Ron
★ 1.1k
38
votes
14
replies
27k
views
9 follow
Filtering Fasta Sequences By Chromosomes Names From A Big Fasta File
fasta
filter
chromosome
updated 7 months ago by
jixiangj
▴ 30 • written 11.2 years ago by
sthait
▴ 120
15
votes
8
replies
27k
views
How to generate a fasta file from a python dictionary or lists?
sequence
fasta
list
dictionary
python
updated 8 months ago by
Ram
40k • written 8.4 years ago by
grayapply2009
▴ 280
77
votes
86
replies
27k
views
20 follow
Tool:
Introducing Clumpify: Create 30% Smaller, Faster Gzipped Fastq Files. And remove duplicates.
clumpify
storage
bbmap
pigz
compression
updated 3 months ago by
Ram
40k • written 6.8 years ago by
Brian Bushnell
18k
72
votes
71
replies
27k
views
17 follow
Tool:
EnhancedVolcano: Publication-ready volcano plots with enhanced colouring and labeling
volcano-plot
bioconductor
ggplot2
ggrepel
updated 3 months ago by
Ram
40k • written 5.1 years ago by
Kevin Blighe
86k
36
votes
23
replies
27k
views
11 follow
bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open shared object file: No such file or directory
bcftools
updated 28 days ago by
s.w.vanderlaan
▴ 40 • written 2.2 years ago by
Michal Nevo
▴ 110
57
votes
19
replies
27k
views
6 follow
Forum:
Attention: Bowtie2 And Multiple Hits
next-gen
bowtie
mapping
updated 7 months ago by
Ram
40k • written 11.0 years ago by
David Langenberger
10k
26
votes
13
replies
27k
views
8 follow
Bowtie2 parameters for best alignment
alignment
Assembly
genome
SNP
updated 6 months ago by
Medhat
9.7k • written 9.3 years ago by
cvu
▴ 180
14
votes
6
replies
27k
views
7 follow
How To Convert Bed Format To Gtf?
gtf
bed
updated 7 weeks ago by
alejandrogzi
▴ 30 • written 10.6 years ago by
ftp
▴ 140
53
votes
11
replies
26k
views
10 follow
Out Of Disk Space With Picard Tools ?
picard
updated 3 months ago by
Ram
40k • written 11.5 years ago by
Gabriel R.
★ 2.9k
6
votes
5
replies
26k
views
Bcftools view options for SNPs calling.
bcftools
samtools
SNP
mpileup
updated 10 months ago by
Ram
40k • written 8.2 years ago by
Eddie_I
▴ 30
19
votes
15
replies
26k
views
6 follow
tophat-cufflinks command pipeline
cufflinks
tophat
updated 8 months ago by
Ram
40k • written 8.8 years ago by
biolab
★ 1.4k
45
votes
15
replies
26k
views
15 follow
How To Do Normalization Of Two Chip-Seq Data?
chip-seq
updated 7 months ago by
baijiangshan9726
• 0 • written 11.5 years ago by
yatung
▴ 200
55
votes
23
replies
26k
views
11 follow
Remove duplicates in fasta file based on ID
fasta
sort
sed
updated 8 months ago by
Ram
40k • written 8.4 years ago by
oliver.bayfield
▴ 210
71
votes
26
replies
26k
views
15 follow
Tutorial:
How To Separate Illumina Based Strand Specific Rna-Seq Alignments By Strand
RNA-seq
updated 7 months ago by
Ram
40k • written 9.6 years ago by
Istvan Albert
98k
13
votes
3
replies
26k
views
What is the difference between blastx and tblastn?
assembly
blast
ncbi
sequence
updated 4 months ago by
Ram
40k • written 4.3 years ago by
Kumar
▴ 120
28
votes
7
replies
26k
views
7 follow
Extracting List Of Genes Associated With A Pathway In Kegg
pathway
updated 9 months ago by
Ram
40k • written 10.5 years ago by
Dataminer
★ 2.8k
18
votes
14
replies
26k
views
7 follow
removing batch effects using ComBat and SVA
R
ComBat
SVA
Batch effect
updated 5 weeks ago by
maximal_life
▴ 20 • written 7.3 years ago by
LJ
▴ 260
63
votes
27
replies
26k
views
18 follow
Data Too Big To Be Stored In Memory: Common Options
python
updated 7 months ago by
Ram
40k • written 10.9 years ago by
Click downvote
▴ 720
49
votes
28
replies
26k
views
12 follow
Trimming A Fastq File Representing Rna-Seq Data
fastq
RNA-seq
updated 6 months ago by
Ram
40k • written 11.5 years ago by
Varun Gupta
★ 1.3k
24
votes
22
replies
26k
views
11 follow
replace fasta headers with another name in a text file
perl
updated 12 weeks ago by
nr299
• 0 • written 9.3 years ago by
Jemo
▴ 60
46
votes
14
replies
26k
views
12 follow
Which Bioinformatics Tools Are Written In Python
python
updated 3 months ago by
Ram
40k • written 11.2 years ago by
Chen Sun
★ 1.1k
66
votes
6
replies
26k
views
8 follow
What Is A "Spot" In Sra Format
sra
fastq
format
updated 8 months ago by
Ram
40k • written 12.1 years ago by
Daniel Standage
4.1k
32
votes
15
replies
25k
views
8 follow
Kegg Data Download
kegg
pathway
api
updated 11 months ago by
Ram
40k • written 10.4 years ago by
anika.menon22
▴ 190
1,000 results • Page
4 of 20
Recent Votes
A: running RAxML and MrBayes over concatinated multiple sequence alignments
Comment: Weirdness in annotation (missing allele frequencies)
Comment: Weirdness in annotation (missing allele frequencies)
Comment: ncbi error report log for validate fastq issue
Comment: ncbi error report log for validate fastq issue
A: Bowtie2 MAPQ difference using local vs end-to-end alignment
C: Bowtie2 MAPQ difference using local vs end-to-end alignment
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Recent Replies
Comment: Weirdness in annotation (missing allele frequencies)
by
Can Abdullah
• 0
Yes, I was confused as well. I think the same. Maybe Annovar uses some other resources besides gnomad, but it does not make sense. Also, I …
Comment: Weirdness in annotation (missing allele frequencies)
by
Can Abdullah
• 0
Thank you so much, I decided to move forward as you suggested. Initially, I thought using the most up-to-date databases and positions would…
Comment: Getting the overlap between two GTF files
by
GenoMax
134k
Something in `AGAT` should work: https://agat.readthedocs.io/en/latest/?badge=latest
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Pierre Lindenbaum
157k
> My problem has been with trying to loop the process. ok here is a **nextflow** based solution,(NOT tested) workflow { …
Comment: Assessing Rockhopper's output
by
langziv
▴ 50
I see. I tried using the GUI Rockhopper for RNA-seq analysis of a K. pneumoniae strain, but the program didn't find the strain's assembly…
Answer: Getting the overlap between two GTF files
by
Pierre Lindenbaum
157k
bedtools intersect \ -a <(awk '/^[^#]/ {printf("%s\t%d\t%s\t%s\n",$1,int($4)-1,$5,$0);}' file1.gtf | sort -t $'\t' -k1,1 -k2…
Comment: Assessing Rockhopper's output
by
shelkmike
▴ 980
I used Rockhopper only for bacterial genomes assembled into circular contigs or circular scaffolds.
Comment: Unable to install HorvathMammalMethylChip40manifest packages
by
ATpoint
77k
There is no package of that name in CRAN or Bioconductor. Where do you have it from, so which tutorial you follow?
Comment: Subclustering of intergated cells from scRNA-seq data
by
fifty_fifty
▴ 60
thank you. This is exactly what I was looking for
Answer: Search RCSB with a list of protein names?
by
Mensur Dlakic
★ 25k
This is not the most elegant solution, but it should work with previous suggestions. In this remote directory: http://ftp.wwpdb.org/p…
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Ram
40k
The manual says you can split samples out at once using the `+split` plugin. So, `bcftools +split -Oz -o <PATH> file.vcf.gz` should do the …
Comment: differences between trajectories in conditions with Monocle3 or other tools
by
Amitm
★ 2.2k
Phate (or Monocle) doesn't do UMAP. If you have done UMAP already (using seurat) then you could use the cell barcode to UMAP cluster label …
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I figured out how to make it work. The bams have to be comma separated not just by space: cd "${input_directory_with_associated_bam_fi…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
Hey, this is so close to what I'm looking for - I clarified in my edit. This is a great way to batch download lots of PDBs in RCSB by their…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
This is a really neat package, but I don't think it does exactly what I want, so I've clarified my question. This seems like it grabs a PD…
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