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1,000 results • Page
5 of 20
Sort: replies
Rank
Views
Votes
Replies
103
votes
34
replies
113k
views
23 follow
How To Split A Bam File By Chromosome
bam
updated 3 months ago by
Ram
43k • written 11.9 years ago by
GPR
▴ 390
31
votes
34
replies
7.4k
views
11 follow
Forum:
pros- and cons- : programming skills vs. GUI
programming
lecture
updated 13 months ago by
Ram
43k • written 8.4 years ago by
TriS
★ 4.7k
54
votes
34
replies
4.9k
views
13 follow
Forum:
Should We Release Database Dumps Of All Questions On Biostar?
meta
biostars
updated 14 months ago by
Ram
43k • written 13.4 years ago by
Istvan Albert
100k
2
votes
34
replies
6.8k
views
How to install pip on HPC server
software error
6.2 years ago by
Bioinfonext
▴ 460
44
votes
34
replies
13k
views
7 follow
Technical replicates in RNAseq
RNA-Seq
technical_replicates
2.2 years ago by
grant.hovhannisyan
★ 2.6k
16
votes
34
replies
4.8k
views
7 follow
GC percent detection for several number of genes
GC%
large number of genes
7.1 years ago by
nazaninhoseinkhan
▴ 520
147
votes
34
replies
55k
views
17 follow
Tutorial:
Working with MAF files (Mutation Annotation Format) from the TCGA (The Cancer Genome Atlas)
music
cancer
tcga
mutation
maf
updated 14 months ago by
Ram
43k • written 11.0 years ago by
Cyriac Kandoth
6.0k
16
votes
34
replies
12k
views
6 follow
Why am I unable to load my data from a tab separated file into R?
microarray
programming
R
updated 2.0 years ago by
Ram
43k • written 9.2 years ago by
Mo
▴ 920
30
votes
34
replies
6.7k
views
enrichment of gene set with enrichR
enrichR
RNA-Seq
R
updated 6.1 years ago by
WouterDeCoster
47k • written 6.1 years ago by
bikash2510
▴ 30
65
votes
34
replies
27k
views
20 follow
Phylogenetic Analysis Of Whole Genomes
phylogenetics
tree
updated 2.1 years ago by
Ram
43k • written 13.8 years ago by
Aparna
▴ 130
11
votes
34
replies
2.2k
views
De novo transcriptome from PE reads with high multi-mapping rate (Bowtie2)
RNA-Seq
read support
Assembly
bowtie2
3.6 years ago by
Dunois
★ 2.5k
15
votes
34
replies
16k
views
10 follow
Issues installing bcl2fastq?
bcl2fastq
demultiplexing
updated 7 weeks ago by
barslmn
★ 2.1k • written 6.7 years ago by
a.rex
▴ 350
19
votes
34
replies
5.7k
views
6 follow
How can I convert the blast results into a matrix?
perl
linux
python
blast
updated 2.2 years ago by
Ram
43k • written 9.4 years ago by
kandoigaurav
▴ 150
15
votes
34
replies
12k
views
Improving efficiency of awk for large files
awk
updated 18 months ago by
Ram
43k • written 8.8 years ago by
AW
▴ 350
12
votes
34
replies
5.1k
views
Hidden Markov Models Within Sequence Analysis -- Dispelling Misconceptions + Fixing Explanations
sequencing
updated 12.1 years ago by
Fabian Bull
★ 1.3k • written 12.6 years ago by
Delinquentme
▴ 200
172
votes
34
replies
26k
views
29 follow
Recommend Your Favorite Introductory "R In Bioinformatics" Books And Resources
r
books
bioconductor
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Michael
54k
143
votes
33
replies
30k
views
20 follow
C/C++ Libraries For Bioinformatics?
c
c
updated 5.0 years ago by
chernouhov sergey
▴ 50 • written 13.9 years ago by
User 59
13k
29
votes
33
replies
8.1k
views
12 follow
getBM error while using bioMart
biomart
getBM
ensembl
updated 3.5 years ago by
user17394
• 0 • written 3.6 years ago by
MatStat
▴ 160
48
votes
33
replies
43k
views
16 follow
Extract A Group Of Fasta Sequences From A File
fasta
python
perl
biopython
bioperl
updated 2.4 years ago by
Ram
43k • written 13.8 years ago by
Kyle
▴ 60
138
votes
33
replies
66k
views
19 follow
VCF files: Change Chromosome Notation
vcf
next-gen
sequence
updated 18 months ago by
Ram
43k • written 10.0 years ago by
Quak
▴ 490
121
votes
33
replies
39k
views
24 follow
Forum:
Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 11 months ago by
carlopecoraro2
★ 2.5k • written 10.7 years ago by
Eric Normandeau
11k
70
votes
33
replies
17k
views
18 follow
Choosing Random Set Of Seqs From Larger Set
random
fasta
updated 24 months ago by
Ram
43k • written 12.1 years ago by
razor
▴ 190
11
votes
33
replies
17k
views
How we can analyze sanger sequence chromatogram?
SNP
sequencing
alignment
sanger
updated 2.2 years ago by
Ram
43k • written 9.5 years ago by
Elnaaz
▴ 40
11
votes
33
replies
4.0k
views
Detect Regions In A List That Are Within 1 Megabase Of Each Other
python
snp
genomics
overlap
updated 12.5 years ago by
Michael
54k • written 12.5 years ago by
Rubal
▴ 350
27
votes
33
replies
3.8k
views
7 follow
Forum:
Biostars question catagories
statistics
biostars
meta
updated 12 months ago by
Ram
43k • written 7.5 years ago by
John
13k
12
votes
33
replies
3.2k
views
Missing sequences in local NT database
nt
blast
ncbi
blastdbcmd
updated 5.6 years ago by
lieven.sterck
15k • written 5.6 years ago by
Prakki Rama
★ 2.7k
41
votes
33
replies
18k
views
10 follow
How To Convert Xml Into A Decent Parseable Format?
xml
parsing
perl
subjective
updated 12.9 years ago by
Jrbeaman
▴ 30 • written 12.9 years ago by
Lyco
★ 2.3k
27
votes
33
replies
17k
views
high % of reads mapped to multiple loci after STAR mapping
RNA-Seq
STAR
single-end
updated 6.4 years ago by
Hussain Ather
▴ 990 • written 6.4 years ago by
Lila M
★ 1.2k
6
votes
33
replies
2.8k
views
I need to understand this image
phylogenies
updated 7.2 years ago by
Emily
23k • written 7.2 years ago by
eng_samar_zayed
• 0
10
votes
33
replies
3.8k
views
Looking for a tool like fastq screen but for ONT data
gridion
nanopore
ont
minion
updated 10 months ago by
Ram
43k • written 5.8 years ago by
Rox
★ 1.4k
50
votes
33
replies
55k
views
10 follow
I would like to add gene names to a volcano plot obtained from DEseq2
RNA-Seq
DEseq2
R
updated 6.0 years ago by
Kevin Blighe
87k • written 7.8 years ago by
ta_awwad
▴ 340
16
votes
33
replies
8.4k
views
9 follow
Back-filling missing genotypes in merged VCF
vcf
updated 16 months ago by
Pierre Lindenbaum
161k • written 9.5 years ago by
Katie D'Aco
★ 1.1k
298
votes
33
replies
108k
views
25 follow
Tutorial:
Cheat Sheet For One-Based Vs Zero-Based Coordinate Systems
coordinates
updated 14 months ago by
Ram
43k • written 10.5 years ago by
Obi Griffith
20k
24
votes
33
replies
8.5k
views
Contribute To The Nat Gen Paper "Principles For The Post-Gwas Functional Characterisation Of Risk Loci"
gwas
snp
updated 13.4 years ago by
Khader Shameer
18k • written 13.4 years ago by
Giovanni M Dall'Olio
28k
88
votes
32
replies
9.6k
views
11 follow
Forum:
Vintage / unconventional pictures for Bioinformatics
history
picture
updated 2.1 years ago by
Ram
43k • written 9.5 years ago by
Pierre Lindenbaum
161k
153
votes
32
replies
8.5k
views
23 follow
Picking A Programming Language And Where To Begin
programming
next-gen
sequencing
c
subjective
updated 12.2 years ago by
Palani Kannan
▴ 60 • written 12.7 years ago by
Travis
★ 2.8k
82
votes
32
replies
7.7k
views
13 follow
Forum:
Shall We Go Back To Stackexchange?
meta
updated 14 months ago by
Ram
43k • written 11.4 years ago by
14134125465346445
★ 3.6k
3
votes
32
replies
3.4k
views
fastqc report for degradome reads
fastqc
adaptor
6.1 years ago by
Sam
▴ 150
32
votes
32
replies
8.0k
views
11 follow
Should We Be Using Much More Json In Our Delimited Data Formats?
gff
vcf
updated 13.2 years ago by
Erik Garrison
★ 2.4k • written 13.2 years ago by
Casbon
★ 3.3k
53
votes
32
replies
13k
views
13 follow
Bio-Javascript?
updated 2.0 years ago by
Ram
43k • written 13.2 years ago by
Lee Katz
★ 3.1k
9
votes
32
replies
3.0k
views
Differentially gene expressed analyses of two different samples
RNA-Seq
R
next-gen
rna-seq
updated 7.4 years ago by
EVR
▴ 610 • written 7.4 years ago by
Mehmet
▴ 820
10
votes
32
replies
3.7k
views
6 follow
Why my CD31+ endothelial cells RNAseq didn't express CD31?
CD31
HISAT2
endothelialcells
Stringtie
RNAseq
3.0 years ago by
Youyy
• 0
93
votes
32
replies
33k
views
26 follow
Linux Distros Best Suited For Bioinformatics?
linux
written 12.2 years ago by
Sat3Lite
▴ 150
129
votes
32
replies
129k
views
29 follow
Is There A Free Alternative To Ingenuity Pathway Analysis?
network
pathway
updated 2.1 years ago by
Ram
43k • written 13.1 years ago by
Gon
▴ 540
0
votes
32
replies
9.4k
views
DoHeatmap clusters, Seurat pipeline
scRNA-sequencing
3.6 years ago by
Morris_Chair
▴ 350
9
votes
31
replies
4.9k
views
6 follow
Clustering Million Points : Transcript Start And End Sites
clustering
updated 12.0 years ago by
Khader Shameer
18k • written 12.1 years ago by
Abhi
★ 1.6k
59
votes
31
replies
4.9k
views
12 follow
News:
Example of how bioinformaticians can publish in Scientific Reports (by nature publishing group) using publicly available NGS data
publication
NGS
updated 12 months ago by
Ram
43k • written 7.3 years ago by
David Langenberger
11k
31
votes
31
replies
2.1k
views
collecting ultraconserved elements from RNA-seq data
RNA-Seq
sequence
genome
updated 7.7 years ago by
BioinfGuru
★ 1.7k • written 7.7 years ago by
Farbod
★ 3.4k
120
votes
31
replies
199k
views
17 follow
Correct Way To Parse A Fasta File In Python
python
fasta
updated 5 months ago by
Ram
43k • written 14.0 years ago by
Eric Normandeau
11k
38
votes
31
replies
2.8k
views
10 follow
Forum:
To the Biologists out there: What bioinf tool are you missing?
education
career
updated 12 months ago by
Ram
43k • written 7.8 years ago by
mschmid
▴ 180
1,000 results • Page
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Comment: Ideal PC configurations and operating system for bioinformatics laboratory
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
Comment: Convert SAM to BAM
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Recent Replies
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
BioinfGuru
★ 1.7k
Hi, Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
Comment: Low mapping rate with Salmon
by
i.sudbery
19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.4k
If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value. https://www.b…
Comment: genome assembly records not present in assembly_summary.txt
by
sapuizait
▴ 10
jesus its in the Genbank file and I was looking at the refseq! I m such a moron - thanks for pointing it out -sorry about that :(
Comment: ScRNA data question
by
bk11
★ 2.4k
I wonder how the `Vlnplots` will look if you normalize the data using `NormalizeData()` function in `Seurat`. The flat line in your plot ar…
Comment: Low mapping rate with Salmon
by
GenoMax
141k
> Does that mean that the reads that are not mapped to my trascriptome are not exons/coding genes? Reads are likely aligning in regions wh…
Comment: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
cmdcolin
★ 3.8k
the gencode link is broken now but here is a back up of that blogpost on archive https://web.archive.org/web/20130618221342/http://gencodeg…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
I'll try to make the decoy file again but I remember you also told me that it is only recommended and not mandatory for running `salmon`. I…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
For doing this, should I just look at the log.out.file?
Answer: Heatmap and rna-seq
by
pinheirofabiano
▴ 10
install.packages("pheatmap") library(pheatmap) setwd("/Users/data_analysis/results") data <- read.table(file…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
Thanks for the link GenoMax. But I was wondering, should not alignment and mapping have a similar rate and thus be correlated? In my case I…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
bedtools intersect \ -a <(sort -t $'\t' -k1,1 -k2,2n A.bed) \ -b <(awk '{X=250000;P=int($2);printf("%s\t%d\…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
• 0
The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
Comment: ScRNA data question
by
starswillfade
▴ 10
I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
Comment: ScRNA data question
by
starswillfade
▴ 10
![enter image description here][1] I am already using Seurat V5 I think. I am using SCTransform as it was recommended by the seurat page. I…
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